What is Resolution?
The resolution is the separation of two components in a mixture, calculated by:
Resolution = 2 × (tR2 − tR1)/(W1 + W2)
where tR2 and tR1 are the retention times of the two components; and W2 and W1 are the corresponding widths at the bases of the peaks obtained by extrapolating the relatively straight sides of the peaks to the baseline.
Where electronic integrators are used, it may be convenient to determine the resolution, by the equation:
Resolution = 1.18 × (tR2 − tR1)/(W1,h/2 + W2,h/2)
What relation Resolution and Peak seperations?
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